
Agrofert is a Czech conglomerate holding company headquartered in Prague, Czech Republic. It operates agriculture, food, chemical, construction, logistics, forestry, energy and mass media industries in the European Union and China with over 250 subsidiary companies. Most of them are owned directly by AGROFERT, a.s., the minor part of the portfolio consists of companies in which the maternal company has substantial influence. The companies of AGROFERT, a.s. operate mostly in central Europe: in the Czech Republic, Slovakia, Germany and Hungary. Furthermore, it operates in another 18 countries and 4 continents. In 2012 the consolidated profits of the company have reached 132.5 billion Kč. AGROFERT, a.s. is one of the biggest companies in the Czech Republic. Agricultural companies of the conglomerate manage 386,1 square miles of land in the Czech Republic. Andrej Babiš has directed the conglomerate until January 2014 and owned it until 2017, he was forced to transfer ownership of company to two trust funds AB private trust I and AB private trust II controlled by his family and lawyers in order to comply with the new conflict of interest legislation passed by the Parliament. In 2017 the conglomerate employed 33 thousand people, 22 thousand of them in CR.

Strunal Schönbach s.r.o. is a string instrument manufacturer based in Luby, Czech Republic. Strunal, in its current corporate structure that was established in 1992, is an outgrowth of Cremona, a joint-stock company founded in Schönbach in Czechoslovakia in 1922. In 1946, after World War II, the Cremona cooperative was founded in Luby.

Author is a Czech brand of bicycles, bike accessories and sporting goods. The brand was founded in 1993. Author manufactures bicycles in the following categories road bikes, mountain bikes, cross-country bicycles, touring, freestyle, junior and women's. Author also manufactures e-bikes.

Avast Software s.r.o. is a Czech multinational cybersecurity software company headquartered in Prague, Czech Republic that researches and develops computer security software, machine learning and artificial intelligence. Avast has more than 435 million monthly active users and the second largest market share among anti-malware application vendors worldwide as of April 2020. The company has approximately 1,700 employees across its 25 offices worldwide. In July 2021, NortonLifeLock, an American cybersecurity company, announced that it is in talks to merge with Avast Software. In August 2021, Avast's board of directors agreed to an offer of US $8 billion.

AVG Technologies is a brand of cybersecurity, privacy, performance and utility software applications for desktop computers and mobile devices developed by Avast. AVG was a cybersecurity software company founded in 1991 and it merged into Avast following an acquisition in 2017. It typically offers freeware, earning revenues from advertisers and from users that upgrade to paid versions for access to more features.

Barum is a manufacturer of rubber tyres, based in the Czech Republic.

Becherovka, formerly Karlsbader Becherbitter, is a herbal bitters, often drunk as a digestif. It is produced in Karlovy Vary, Czech Republic by the Jan Becher company. The brand is owned by Diageo. It is made from a secret recipe based on more than twenty types of herbs and spices.

Bílinská kyselka is strongly mineralized alkaline bicarbonate mineral water from Bílina city. Particularly sodium, potassium, calcium, magnesium, and iron are present as cations, as are anions of chloride, sulfate, and bicarbonate. The temperature of spring water ranges between 17 and 20 °C.. Due to the high content of free carbon dioxide is naturally carbonated and packaged without additional chemical modifications. Water is collected from a borehole from a depth of 191 m under the mountain Bořeň. Biliner is available in Europe in one liter and 500 ml bottles. Biliner bottles are cobalt blue polyethylene terephthalate.

České Radiokomunikace is a telecommunications company in the Czech Republic. Company offers complex portfolio of telecommunications and ICT services with their own infrastructure for a wholesale and business customers.

Czech Airlines j.s.c. is the flag carrier of the Czech Republic. Its head office is located in the Vokovice area of Prague's 6th district and its hub is Václav Havel Airport Prague. The company mainly operates scheduled flights, serving 22 destinations as of 2021. Czech Airlines carried 2.26 million passengers in 2016, a 13% increase compared to 2015. Czech Airlines Technics is responsible for aircraft maintenance.

Czech Sport Aircraft, a.s. is an aircraft manufacturer based in the Czech Republic. Czech Sport Aircraft company formally entered the market in 2009.

Fernet Stock is a herbal bitters made in Plzeň-Božkov, Czech Republic. It is flavoured with approximately 14 herbs, imported from the Mediterranean and the Alps. It is also available in a sweeter form as Fernet Stock Citrus. The original Fernet is approximately 40% alcohol, whereas the Citrus is a lower 30%.

Foma Bohemia spol. s.r.o is a photographic company based in Hradec Králové, Czech Republic established in 1921, originally as Fotochema, being renamed in 1995 on privatisation. They are mostly known for their line of black and white films and papers but also produce movie film, X-ray films for medicine and industry and personal dosimetry film along with processing chemicals. They formerly produced aerial and surveillance films.

Frisco is a flavored cider by the Pilsner Urquell Brewery brewery that is available in Czech Republic, Slovakia and Lithuania. It was first introduced in 2004. The drink was manufactured by Lech Browary Wielkopolski in Poznan at first, but since 2013 it is made by Pilsner Urquell Brewery Original recipe included barley malt, but since 2017 the drink is a proper cider made from apples.

Furch Guitars is a Czech manufacturer of premium acoustic guitars and acoustic basses founded in 1981 by František Furch. Furch guitars are claimed to be used by such artists as Al di Meola, Suzanne Vega, Per Gessle, Glen Hansard, and Calum Graham.

Pivovar Holba a.s. is a brewery located in Hanušovice, Czech Republic. The major owner is a brewer company PMS Přerov a.s. Annual production is over 350,000 hectoliters. The most notable product is Holba Šerák, a medium strong beer, named after the nearby mountain. The motto of the brewery is Pure Beer from Mountains.

JAWA is a motorcycle and moped manufacturer founded in Prague, Czechoslovakia in 1929 by František Janeček, who bought the motorcycle division of Wanderer. The name JAWA was established by concatenating the first letters of Janeček and Wanderer. In the past, especially in the 1950s, JAWA was one of the top motorcycle manufacturers and exported its 350 model into over 120 countries. The best known model was the 350 Pérák and in the 1970s the 350 Californian. It appeared in typical black and red coloring from California to New Zealand. After 1990 a significant loss of production occurred. A successor company was formed in 1997 in Týnec nad Sázavou, continuing the name as JAWA Moto.

JetBrains s.r.o. is a Czech software development company who makes tools for software developers and project managers. As of 2019, the company has offices in Prague, Saint Petersburg, Moscow, Munich, Boston, Novosibirsk, Amsterdam, Foster City and Marlton, New Jersey.
The JIHOSTROJ a.s., based in Velesin, Czech Republic, is a company that manufactures hydraulic and aircraft fuel pumps, as well as other components for automotive and aircraft industry. Particularly the FCU, Propeller governor and main fuel pump for Walter M601 turboprop engines. Jihostroj is the majority owner of Jawa Moto company.

Kaden Nachod is the somewhat anglicized name for the Kovodružstvo Náchod toy factory in the town of Nový Hrádek in the Czech Republic. The factory, however, started making toys about 1950 when the country was still communist united Czechoslovakia.
Kaipan is a small Czech vehicle manufacturer in Smržovka focused on producing roadsters.

Kenvelo is a clothing firm originally from the Czech Republic. It currently operates approximately 270 stores in 18 countries: Czech Republic, Croatia, Slovakia, Romania, Israel, Bulgaria, Russia, Austria, United Kingdom, Lithuania, Belarus, Albania, Latvia, Estonia, Malta, Ukraine, Serbia and Germany.

Kofola is a carbonated soft drink produced by Czech company Kofola, headquartered in Ostrava, Czech Republic. It is the principal rival of Coca-Cola and Pepsi in the Czech Republic and Slovakia. The company is one of the leading soft drinks producers and distributors in Central and Eastern Europe.

Koh-i-Noor Hardtmuth a.s. is a Czech manufacturing company of stationery products, based in České Budějovice. Having been established in 1790, it is one of the oldest stationery companies in the world.

Komerční banka (“KB”) is a major Czech bank and the parent company of KB Group, a member of the Société Générale international financial group. KB is a universal bank providing a wide range of services in retail, corporate and investment banking complemented by specialised financial services produced by KB’s subsidiaries or other SG Group companies. KB Group operates in the Czech Republic and also provides services to corporate clients in Slovakia. It serves more than 1.6 million customers in 399 branches.

Aircraft Industries, a.s., operating as LET, n.p., is a Czech civil aircraft manufacturer. Its most successful design has been the L-410 Turbolet, of which more than 1200 units have been built. Its head office is in Kunovice, Uherské Hradiště District. LET has been owned by Russian company UGMK since 2008. The company operates the sixth largest Czech airport and a private secondary school.

Letov is an aircraft company located in Letňany, Prague, Czech Republic. It is the oldest aircraft company in the region.

Merkur refers to a metal construction set built in Czechoslovakia. It was also referred to as Constructo or Build-O in English-speaking countries and Tecc in the Netherlands.
The Moravská gobelínová manufaktura — MGM, is a tapestry manufactory located in the town of Valašské Meziříčí, in the Zlín Region of the Czech Republic. The manufactory has been involved in the area of handmade classical and artistic tapestries, restoring and also creating new pieces with modern themes for more than fifty years. It is considered the first tapestry manufactory in Czechoslovakia, and the only workshop of this kind in Moravia and Czech Silesia.

Moser a.s. is a luxury glass manufacturer based in Karlovy Vary, Czech Republic. The company is known for manufacturing stemware, decorative glassware, glass gifts and various art engravings. Moser is one of the most collected of 20th century decorative glass and has been used everywhere from palaces to local restaurants. From its beginnings in 1857, as a polishing and glass engraving workshop, it developed into a lead-free glass manufacturer lasting through the 20th century until the present. It is considered as the most luxurious Czech brand as well as one of the world's most famous brands of luxury crystal. Every piece of glass that is made by Moser is hand made.

MTX is an automobile company in the Czech Republic, that has been previously in Czechoslovakia engaged in the manufacture of racing and formula cars since 1969. Originally, it was a repair shop for Škoda racers. Currently, MTX is engaged in the development of special vehicle modifications, service activities and veteran renovations. The company now has branches in Prague (headquarters), Plzeň (manufacture) and Mělník (autoservis).

Rückl Crystal a.s. is a Bohemian glass factory situated in the village called Nižbor, about 7 km west from the city of Beroun, Czech Republic. The glass factory produces 24% lead crystal. Major part of its production is focused on export. Some production stays on the market in the Czech Republic.

Prvni brnenska strojirna Velka Bites, a. s. is a Czech engineering and manufacturing company. It focuses mainly on the development, testing, and production of devices for the aircraft industry. Together with PBS Energo, a.s.; PBS Brno, a.s. and PBS Aerospace Inc., PBS VB is a member of PBS GROUP, a.s.

Petrof is a Czech piano manufacturer founded in 1864. It is the leading European piano manufacturer, exporting to more than 60 countries.
Pivovar Broumov is a traditional brewery in Broumov in the eastern Czech Republic. It produces a light beer for domestic consumption and a dark beer for the export to Germany.

Praga is a manufacturing company based in Prague, Czech Republic. The company produced automobiles, karts and planes. The Praga V3S 5-ton truck was used by the Czechoslovak Army for more than half a century.
PRIM is a Czech trademark for a range of watches. The brand is now being used by the two Czech watches manufacturers.

ProFe is a Czech aircraft manufacturer. The company was founded in 1992 by Václav Brandejs and Ivan Brandejs to produce kit aircraft, particularly gliders and motor gliders.

The Prusa i3 series consists of open-source fused deposition modeling 3D printers, manufactured by Czech company Prusa Research under the trademarked name Original Prusa i3. Part of the RepRap project, Prusa i3 printers were named the most used 3D printer in the world. The first Prusa i3 was designed by Josef Průša in 2012, and was released as a commercial kit product in 2015. The latest model is available in both kit and factory assembled versions. The Prusa i3's comparable low cost and ease of construction and modification has made it popular in education and with hobbyists and professionals. Since the i3 series is open source, there have been many variants produced by companies and individuals worldwide.

The Prusa Mini, sometimes stylized as the Original Prusa MINI, is an open-source fused deposition modeling 3D printer that is manufactured by the Czech company Prusa Research. The printer is the lowest cost machine produced by Prusa Research and is designed as a first printer or as part of a 'print farm'.

Rudolf Kämpf is a porcelain manufacturer in the Czech Republic.

Škoda Auto a.s., often shortened to Škoda, is a Czech automobile manufacturer founded in 1895 as Laurin & Klement and headquartered in Mladá Boleslav, Czech Republic.

Škoda Transportation a.s. is a Czech engineering company that continues the legacy of Škoda Works' rolling stock manufacturing that started at the end of 19th century in Pilsen. Following the first world war, the Works commenced locomotive production in a separate factory dedicated to that purpose. This factory became a separate company in the 1990s. Today, Škoda Transportation develops and manufactures electric rail vehicles for urban and railway transport.

Skyleader a.s. is a Czech aircraft manufacturer based in Jihlava. The company specializes in the design and manufacture of ultralight aircraft in the form of ready-to-fly aircraft in the Fédération Aéronautique Internationale microlight and American light-sport aircraft categories.

SOR Libchavy is a Czech manufacturer of buses for urban, intercity and tourist traffic and trolleybuses. It was established in Libchavy in 1991.

Strunal Schönbach s.r.o. is a string instrument manufacturer based in Luby, Czech Republic. Strunal, in its current corporate structure that was established in 1992, is an outgrowth of Cremona, a joint-stock company founded in Schönbach in Czechoslovakia in 1922. In 1946, after World War II, the Cremona cooperative was founded in Luby.

Supraphon Music Publishing is a Czech record label, oriented mainly towards publishing classical music and popular music, with an emphasis on Czech and Slovak composers.

Tatra is a Czech vehicle manufacturer in Kopřivnice. It is owned by the Tatra Trucks company, based in Ostrava, and is the third oldest company in the world producing cars with an unbroken history. The company was founded in 1850 as Ignatz Schustala & Comp., in 1890 renamed in German Nesselsdorfer Wagenbau-Fabriksgesellschaft when it became a wagon and carriage manufacturer. In 1897, Tatra produced the first motor car in central Europe, the Präsident automobile. In 1918, it changed its name to Kopřivnická vozovka a.s., and in 1919 changed from the Nesselsdorfer marque to the Tatra badge, named after the nearby Tatra Mountains on the Czechoslovak-Polish border.

Tatranky are the Czechoslovakia five-layer wafers with chocolate coating only on narrower edges. They were introduced in 1945.

TEDOM a.s is a Czech engineering company which was established by Ing. Josef Jeleček in Třebíč in 1991.TEDOM is an abbreviation of the Czech words TEplo DOMova [The warmth of home]. The primary scope of business of the company is the development and production of CHP units with gas combustion engines. Aside from CHP units, the company's product portfolio also includes combustion engines, all of them designed by TEDOM.

Orlen Unipetrol is a Czech joint stock company owned by a Polish oil company PKN Orlen. The company is engaged in crude oil processing and the production, distribution and sales of fuels and petrochemical products – mainly plastics and fertilisers – in the Czech Republic and the Central European region. It is the only crude oil processing company in the Czech Republic and its earnings rank it among the ten largest Czech companies. It was founded in 1994, and in 2004, it became part of the PKN Orlen group, which owns 100% shares in the company. The following companies have been gradually incorporated into Unipetrol: Kaučuk, Chemopetrol, Benzina, Paramo, Koramo(merged with Paramoin 2003), Česká rafinérská, Unipetrol Trade, Spolana, and Unipetrol Rafinérie. The Unipetrol group includes refineries and production plants in Litvínov and Kralupy nad Vltavou, Paramo in Pardubice and Kolín with its Mogul brand and Spolana in Neratovice, and two research centres in Litvínov and Brno. The network of Benzina fuel stations – the largest network in the Czech Republic with its 406 stations – is also part of Unipetrol.

Y Soft Corporation is a multinational software and electronic hardware company founded in 2000 operating in 17 countries. The company's headquarters are in Brno, Czech Republic, with other offices in France, Hungary, Israel, United Kingdom, United Arab Emirates, United States, Japan, Singapore, Australia, and China.

Zaječická hořká is strongly mineralized natural bitter water from the village of Zaječice in the Ústí nad Labem Region of the Czech Republic.

Zentiva is a pharmaceutical company based in Prague, Czech Republic. The company is developing, manufacturing and marketing a wide range of generic and OTC products.

Zetor is a Czech agricultural machinery manufacturer. It was founded in 1946. The company is based in Brno, Czech Republic. Since June 29, 2002, the only shareholder is a Slovak Company. HTC Holding. The company manufactures tractors and tractor components.