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Alepidomus evermanni is a freshwater species of silverside endemic to western Cuba. This species grows to 3.3 cm (1.3 in) in standard length. It is the only known member of its genus. This species was described as Atherina evermanni by Carl H. Eigenmann in 1903 with a type locality of San Cristobal, Cuba. The specific name honours the American ichthyologist Barton Warren Evermann (1853-1932).

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The barred topminnow is a species of poeciliid fish endemic to Cuba where it prefers patches of aquatic vegetation in ponds. Females grow to a standard length of up to 3.4 cm (1.3 in), while males only reach 2 cm (0.8 in).

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The Cuban gambusia is a species of freshwater fish. It is a member of the family Poeciliidae of order Cyprinodontiformes, and is the type species of its genus. It is native to Cuba, inhabiting primarily stagnant lakes or ponds and slow-moving streams. A carnivorous surface feeder, it occurs in shoals near the shoreline.

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The Cuban gar is a fish in the family Lepisosteidae. It is a tropical, freshwater species, although it also inhabits brackish water. It is found in rivers and lakes of western Cuba and the Isla de la Juventud. The flesh of the fish is edible, but the eggs are poisonous for humans.

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Girardinus falcatus, the goldbelly topminnow, is a species of Cuban tropical fish. Another common name of the species is yellow belly. The fish is pale gold and has a bright blue iris. Males are smaller than females and constantly mate.

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Girardinus metallicus is a species of fish from the family of the Poeciliidae.

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Lucifuga subterranea, or the Cuban cusk-eel, is a species of cavefish in the family Bythitidae. It is endemic to Cuba. Within the caves, sinkholes and crevices in which it occurs it is common, it feeds on cirolanid isopods.

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The Mangrove gambusia is a tropical poeciliid fish species with a restricted, disjunct range one in northwestern Cuba, the other in southeastern Florida. The Florida population has been recently listed as "biologically vulnerable" by the Florida Fish and Wildlife Conservation Commission.