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Božena Benešová, née Zapletalová, was a Czech author and poet whose work is considered to have been at the forefront of psychological prose. The greater part of her youth was spent in Uherské Hradiště and Napajedla, where in 1896 she married a railway clerk named Josef Beneš. In 1908 she and her husband moved to Prague.

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Tereza Boučková is a Czech writer.

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Radka Denemarková is a Czech novelist, dramatist, TV screenplay writer, translator, essayist.

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Eva Hudečková is Czech actress, playwright and writer.

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Halina Pawlowská is a Czech playwright, short story writer, journalist and editor. She has worked as a screenwriter and show presenter for Czech television.

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Eliška Pešková (1833–1895) was a major Czech stage actor and playwright. She published about 50 plays, many of which were translated to other languages. She belonged to the leading stage actors of her time and many of the next generation Czech actors were influenced by her. She is most remembered for her roles as heroines in romantic drama. She was married to the dramatist Pavel Švanda ze Semčic.

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Magdaléna Platzová is a Czech writer.

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Gabriela Preissová, née Gabriela Sekerová, sometimes used pen name Matylda Dumontová, was a Czech writer and playwright. Her play Její pastorkyňa was the basis for the opera Jenůfa by Leoš Janáček, as well as a film by Miroslav Cikán. Her earlier opera The Beginning of a Romance was also based on one of her stories.

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Magdalena Dobromila Rettigová was Czech writer known for her famous cookery book.

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Olga Scheinpflugová was a Czech actress and writer. She was a daughter of the writer, journalist and playwright Karel Scheinpflug. In 1935, she married the writer Karel Čapek.

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Helena Štáchová was a Czech puppeteer, voice actress and playwright. Štáchová served as the head and director of the Spejbl and Hurvínek Theater (S+H), which opened in 1930 as the former Czechoslovakia's first professional puppet theater, from 1996 until her death in 2017. She also provided the voice of popular puppets, Manicka and Mrs. Katerina, who are the female equivalents of the Spejbl and Hurvínek puppet characters. In addition, Štáchová worked as a voice actress as well. Notably, Štáchová provided the Czech language-voice of Lisa Simpson on the Czech version of the American animated sitcom, The Simpsons.

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Magdalena Wagnerová is a Czech writer.