
The Annunciation is a 157 by 315 cm fresco fragment by Correggio, dating to around 1524-1525 and now in the Galleria nazionale di Parma.

Assumption of the Virgin is a 1524-1526 oil on canvas painting by Moretto da Brescia, displayed as the high altarpiece in the Old Cathedral in Brescia.

The Choice of Hercules or Hercules at the Crossroads is a c.1520-1525 oil on canvas painting by Domenico Beccafumi, now in the Museo Bardini in Florence. It is strongly influenced by Baldassarre Peruzzi's frieze at the Villa Farnesina, placing it after Beccafumi's possible trip to Rome around 1512.

Death of Cleopatra is a c.1525 oil on panel painting by Rosso Fiorentino, now in the Herzog Anton Ulrich-Museum in Brunswick.

The Doria Madonna is a c.1525 oil on panel painting by Parmigianino, now in the Galleria Doria-Pamphili in Rome. Its shape and dimensions show it to form a diptych with the Nativity with Angels in the same gallery. A smaller autograph version also survives in the Uffizi.

Lament over the Dead Christ is a 1524 or 1525 oil on canvas painting by Andrea Previtali, produced for the church of Sant’Andrea, Bergamo, where it still hangs.

Life of the Virgin or Scenes from the Life of the Virgin Mary is a 1525 cycle of frescoes by Lorenzo Lotto in the chapel to the left of the Cappella Maggiore in San Michele al Pozzo Bianco in Bergamo - it was the last work the artist produced in the city. Outside the chapel is a work showing The Visitation, whilst the main four paintings of the cycle show The Birth of the Virgin, The Presentation in the Temple, Betrothal of the Virgin and The Annunciation. Above these are a dome and four pendentives showing the Evangelists.

The Lovers, Two Lovers or Love Scene is an oil painting by Giulio Romano, originally painted on panel and later transferred to canvas by A Mitrokin in 1834. Since it was poorly stored and kept off public display due to its subject matter until 1920, it has significant damage, including three large breaks in the canvas sealed with coarse plasters on the reverse and other damage to the paint and varnish layer both before and after the transfer It now hangs in the Hermitage Museum.

The Madonna of the Basket or the Madonna della Cesta is a 1525 painting by Antonio Correggio. As of 2015, it is in the National Gallery, London.

Nativity with Angels is a c.1525 oil on panel painting by Parmigianino, now in the Galleria Doria-Pamphili in Rome. Its shape and dimensions show it to form a diptych with the Doria Madonna in the same gallery.
The Palazzo Bindi Sergardi, previously Agostini and later Casini-Casuccini is, from the outside, a non-descript urban building located on Via dei Pellegrini # 18 in central Siena, region of Tuscany, Italy. The site is best known for containing frescoes by the late-Renaissance painter Domenico Beccafumi and Cristoforo Roncalli.
Florence Charterhouse is a charterhouse, or Carthusian monastery, located in the Florence suburb of Galluzzo, in central Italy. The building is a walled complex located on Monte Acuto, at the point of confluence of the Ema and Greve rivers.

Saint Francis Receiving the Stigmata is an oil on canvas painting by Titian, now in the Museo regionale Agostino Pepoli in Trapani, Sicily. Its treatment of colour has led some to date it to the 1530s or 1550s, but the usual dating is c.1525.

Supper at Emmaus is a 1525 oil on canvas painting by Pontormo and now in the Uffizi in Florence. It is one of the smallest works signed and dated by the artist, in this case on the abandoned scroll in the foreground.

The Temptation of Saint Anthony or The Torment of Saint Anthony is a c.1521-1525 oil on panel painting by Giovanni Gerolamo Savoldo, now in the Timken Museum of Art in San Diego. It may relate to the same artist's The Temptation of Saint Jerome on a similar theme.

The Temptation of Saint Jerome is a c.1521-1525 oil on panel painting by Giovanni Gerolamo Savoldo, now in the Pushkin Museum in Moscow. It may relate to the same artist's The Temptation of Saint Anthony.

Venus with Mercury and Cupid, The School of Love or The Education of Cupid is a c. 1525 painting by the Italian painter Correggio, now in the National Gallery.