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Allegory of Music is a painting by Dosso Dossi, executed in the 1530s and now in the Museo Horne in Florence. It was restored in 1993.

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The Carnesecchi Triptych was an altarpiece of c. 1423–1425 by Masolino, Masaccio and others, depicting the Madonna and Child with Saints Catherine of Alexandria and Julian the Hospitaller. It seems to mark the beginning of Masolino and Masaccio's collaboration.

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Deposition of Christ, also known as Descent from the Cross, is a painting from approximately 1491 by Benozzo Gozzoli. Among Gozzoli's last works, it was still unfinished in his studio by the time of his death. Years later his heirs sold it to the Diocese of Pistoia. It then passed to the Sozzifanti family, from whom it was later inherited by Charles Borbone, Duke of Lucca. It was bought by Herbert Horne in 1907 and now in the Museo Horne in Florence. It was restored in 1990.

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Deucalion and Pyhrra is an oil painting on panel of c. 1520–1525 by Domenico Beccafumi, now in the Museo Horne in Florence.

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Holy Family with the Infant John the Baptist and a Donor is an oil painting on panel by Domenico Beccafumi, with 140 cm of diameter, executed c. 1528, now in the Museo Horne in Florence.

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Life of Esther or Scenes from the Story of Esther is the title of a series of six panel paintings by Sandro Botticelli and Filippino Lippi, showing scenes from the story of Esther and produced in the 1470s. They originally decorated the sides of a pair of cassoni or marriage chests, the two long panels on the fronts, and the smaller ones on the ends. They are now split between five museums in Europe and Canada.

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Pietà is a tempera on wood painting by Filippo Lippi, executed c. 1430-1435, in the Museo Horne, Florence.

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Putti Bearing a Tondo showing the Drunkenness of Noah is a painting by Domenico Beccafumi, executed c. 1522-1523.

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Saint Catherine of Alexandria is a tempera on panel painting, created c. 1512 by Luca Signorelli. It is a fragment from the predella of a lost altarpiece. It is now in the Museo Horne in Florence.

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Saint Jerome in Meditation is an oil painting on panel by Piero di Cosimo, dating to c. 1495–1500 and now in the Museo Horne in Florence.

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Saint Stephen is a panel painting by Giotto, dating to around 1330–1335. It is painted in tempera on gold ground. It is in the collection of and serves as the logo of the Museo Horne in Florence.

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The Monticchiello Altarpiece is a theoretical altarpiece of c. 1315 by Pietro Lorenzetti for the parish church of Santi Leonardo e Cristoforo in Monticchiello, Tuscany. It is thought to have consisted of the Monticchiello Madonna, Saint Margaret or Saint Agatha and Saint Leonard or Benedict, Saint Catherine of Alexandria and Saint Agatha or Saint Margaret.