
The Rio Negro, or Guainía as it is known in its upper part, is the largest left tributary of the Amazon River, the largest blackwater river in the world, and one of the world's ten largest rivers by average discharge.

The Ariau Towers is an abandoned boutique hotel, 60km northwest of Manaus, Brazil, on the Rio Negro, a major tributary of the Amazon River. The concept for the property was that of Dr. Francisco Ritta Bernardino. The property featured 6 towers, with all 291 rooms elevated from the rain forest floor by approximately 10-20m and connected by approximates 5 miles (8.0 km) of catwalks. Amenities included two restaurants and three swimming pools.

The Northern Amazon Ecological Corridor is a proposed ecological corridor connecting conservation units and indigenous territories in the north of the Amazon rainforest of Brazil.

Pico da Neblina National Park is a national park in the state of Amazonas in the north of Brazil, bordering on Venezuela. It overlaps with several indigenous territories, which creates tensions over land use, as does the military presence due to the border location. The park includes lowlands around the Rio Negro, partly flooded, and mountains that include the highest peak in Brazil, after which the park is named. The wide variety of physical environments supports great biodiversity, including several endangered species.
Project Piaba is a fishery initiative located on the Rio Negro tributary of the Amazon river. The program both promotes and researches sustainable aquarium pet fish collection and its impact on the environment. The name of the project comes from the Brazilian Portuguese word, piaba, which means "little fish", referring specifically to the cardinal tetra. Project Piaba is an ongoing project with annual research expeditions to the Rio Negro region. Because of the sustainable nature of the project, its slogan is "Buy a Fish, Save a Tree!"

The Tarumã Açu River is a river in the state of Amazonas, Brazil. It is a left tributary of the Rio Negro, which it enters just west of the city of Manaus.

The Tarumã Mirim River is a river in the state of Amazonas, Brazil. It is a left tributary of the Rio Negro, which it enters west of the city of Manaus.